Human NPY (Neuropeptide Y) ELISA Kit HLP3 Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Bovine BTN1A1

Mouse IgG3 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IgG3

BATF present in the sample is added and binds to antibodies coated on the wells

After 30 minutes incubation and the last washing step

Human NPY (Neuropeptide Y) ELISA Kit HLP3 Substrate solution is then addedThe Human NPY (Neuropeptide Y) ELISA Kit measures NPY in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti NPY antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.