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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken IL12
Then a biotinylated detection antibody specific for Human TIMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The OD value is proportional to the concentration of Mouse LEP
then added a HRP-conjugated antibody specific to Mouse E2 to each microplate well and incubated
Human Carboxypeptidase B (CPB1) ELISA Kit Packaging:96 wells Standards or samples are addedThis ELISA kit is designed to detect Human Carboxypeptidase B (Human CPB1). The assay plate has been pre coated with mouse anti Human Carboxypeptidase B1 monoclonal antibody. When the sample containing Carboxypeptidase B1 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti Human Carboxypeptidase B1 Antibody is added to the wells and binds to Carboxypeptidase B1 in the sample.
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